| Customization: | Available |
|---|---|
| Warranty: | for 12months |
| Brand Name: | Happycare |
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HC-B016 Lab equipment Chemiluminescence immunoassay analyzer CLIA analyzer in a low price/immune hormones analyzer
Features for CLIA analyzer:
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Technical parameters:
Sample form: 96 holes or bars
Linear range: 100-2000K relative light units |
The system and its reagent sets are for ultra-microanalysis, quantitative determination and qualitative analysis of the chemical compositions of virus antigen, antibody, hormone, peptide, tumor protein, metabolite and medicine in serum and other body fluid.
2,Applications & Principles
Technical Theory:
Enzyme-labeled Chemiluminescence Immunoassay Technology
Test Procedure:
1) Sandwich ELISA:Solid Phase Chemiluminescence Enzyme Immunoassay, taking micro plate as its carries, follows the sandwich methodology. The methodology uses 2 specific antibodies which recognize discrete sites on the unknown molecule. The first antibody is precoated onto the wells of the micro plate. It captures antigen in the blood serum after being added into the sample. The other antibody, connected with HRP, is allowed to bind to the antigen. Thus, the immune compounds of antibody -antigen- labeled antibody come into being on the surface of the micro plate. After unbound antigen and HRP-antigen-antibody are removed with a wash by solution, chromogenic substrate solution is added to determine the relative luminescence intensity. According to the relative luminescence intensity of the known antigen concentration of the calibration materials, standard curve is drawn and regression line is available via dual-logarithm mathematical model. Then, the concentration of antigen present in the unknown sample can be calculated.
2)Competitive ELISA: Solid Phase Chemiluminescence Enzyme Immunoassay, taking micro plate as its carries, follows the competitive methodology. Antigen is coated in advance onto the wells of the micro plate. After the test sample is added, free antigen from the test sample and the HRP-labeled antigen compete for the immobilized antibody-binding sites on the wells. Thus, the immune compounds consisting of antibody-antigen and antibody-HRP- labeled antigen come into being on the surface of the micro plate. After unbound antigen and HRP-antigen are washed off by solution, pre-set chromogenic substrate solution is added to determine the relative luminescence intensity. The more the free antigen in the sample, the less the amount of conjugated HRP-antigen. According to the known concentration of the calibration materials and its related luminescence intensity, dosage-reaction curve is drawn and regression line is available after the fitting process. Then, the concentration of antigen present in the unknown sample can be calculated.
3,Specifications
1) Hardware System-X-axis & Y-axis Plane Drive System
Composed of X-axis sensor, Y-axis sensor, X-axis stepping motor control, X-axis drive mechanism and 96-well micro plate pallet. Its function is to cause the plane movement of the 96-well micro plate so as to make each sample well exactly orient to test site.
2) Hardware System-System Control
Composed of Y axis motor control, low ripple power supply, three-dimensional drive control, photon counting and operation, as well as communication interface. It is in charge of movement control, data processing and data transmission.
3) Hardware System- Photon Read
Composed of such cells transferring, as optical fiber movement, photon accelerating, high-voltage dispatching and signal pretreatment. Its function is to realize the optical isolating, photon transferring, light-to-electric conversion and signal-figure processing.
4,Operation Condition
Ambient Temperature:15~35ºC
Relative Humidity:≤85%
Power Supply: 220V±22V;50Hz±1Hz
5,Hardware System-Embedded Computer System(Only for ECLIA-IIM Series )
Made up of industry control embedded system. Its function is to implement display, data input, instrument control, fitting operation, database, report forms printing, etc.
Pictures for HC-B016 CLIA analyzer:
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